Our team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
No, it is not necessary to quantify libraries before Normalase™ I enzyme if the libraries are all known to meet the 6 nM or 12 nM yield threshold. For example, if your library preparation and your standard nucleic acid sample inputs consistently yield 20 nM, quantification can be skipped.
Quantification is only required when library yields are unknown, and potentially when they are under the 6 nM or 12 nM minimum threshold.
For quality control purposes, we recommend quantifying libraries after Normalase PCR using a validated qPCR assay or fluorometric quantification assay.