Our team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
IDT offers a free online calculator, the OligoAnalyzer™ Tool, that will determine the Tm of your oligonucleotide sequence for reaction conditions you specify.
Be sure to include the Mg2+ and dNTP concentrations you plan to use in your reaction, as this will affect the Tm for your oligo.