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Only one included region may be defined per design in the PrimerQuest design tool. The included region is entered as a range; for example, 1200−1500. Multiple excluded regions are allowed per design in PrimerQuest. The "Excluded Region List" is a comma separated list of ranges. For example, 1200−1500, 1600−1700, 1850−1950.
Find both of these parameter fields on the main page of the PrimerQuest design tool under the "Custom Target Region" section. Read more about how to use the PrimerQuest tool here.