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Below are the recommended minimum PCR cycles for direct sequencing of libraries prepared from high-quality genomic DNA for each xGen DNA Library Prep Kits.
When using indexing primer kits, a minimum number of 3 PCR cycles is required to complete adapter sequences, irrespective of whether enough library is available after ligation.
For samples of compromised quality, additional cycles may be required.
xGen DNA Library Prep Kit EZ
| Input material (ng) | Minimum PCR cycles |
|---|---|
| ≥100 | 3 |
| 25 | 5 |
| 10 | 7 |
| 1 | 10 |
xGen DNA Library Prep Kit MC
| Number of cycles required to generate | ||
|---|---|---|
| Input material (ng) |
100 ng library | 1 µg library |
| 1000 | 0* | 2 |
| 500 | 0* | 2–4 |
| 250 | 0–2* | 4–6 |
| 100 | 2–3 | 6–7 |
| 50 | 3–5 | 7–8 |
| 25 | 5–7 | 8–10 |
| 10 | 7–9 | 11–13 |
| 5 | 9–11 | 13–14 |
| 2.5 | 11–13 | 14–16 |
| 1 | 13–15 | 17–19 |