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No. PrimeTime™ Gene Expression Master Mix is compatible with any 5' to 3' exonculease assay or hybridization-based probe assay (such as all Custom PrimeTime qPCR Assays or Presdesigned PrimeTime qPCR Sequences from IDT and TaqMan® Assays from Thermo Fisher Scientific).
SYBR is a registered trademark of Life Technologies, Inc.
EvaGreen dye is a registered trademark of Biotium.