Frequently asked questions

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What cycling parameters will give me the best results when using the PrimeTime™ One-Step RT-qPCR Master Mix?

The master mix can be used with either standard or fast cycling conditions, depending on what qPCR system you are using. For best results, use one of the cycling parameters provided in the PrimeTime One-Step RT-qPCR Master Mix protocol:

  • Standard cycling conditions: 15 min. 50°C; 3 min. 95°C; 35–45x (15 sec. 95°C, 1 min. 60°C).
  • Fast cycling conditions: 15 min. 50°C; 3 min. 95°C; 35–45x (5 sec. 95°C, 30 sec. 60°C).

Categories:
qPCR & PCR

Tags:
master mix qpcr qpcr master mix