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The xGen HS EGFR Pathway Amplicon Panel helps to enable variant calling at and below 1% frequency, with allele frequency dependent on the amount of input material available, and the depth of sequencing.
Using 10 ng of input DNA, the xGen HS EGFR Pathway Amplicon Panel consistently identifies variants at 0.5%, with a recommended sequencing depth of at least 60,000x (1M reads), before deduplication with UMIs and 0.25% from 20 ng of input DNA.
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