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Editing rates and HDR efficiency can vary significantly from system to system and target to target. Strategies for increased HDR efficiency can include using a guide RNA with robust editing activity, adding the Alt-R™ HDR Enhancer V2, and optimizing donor template design. Some troubleshooting measures are to include a positive control, increase the number of clonal isolates screened, and ensure that the intended edit is not lethal, or being selected against.
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