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The rhAmpSeq CRISPR Data Analysis Tool is capable of single-plex and multiplex analyses.
In singleplex analysis, the sample library has only one amplicon that is used to assess the editing of a single site in a sample. You would use singleplex analysis to assess numerous guide RNAs for on-target activity.
In multiplex analysis, the sample library is generated by multiplex PCR and is composed of multiple amplicons that assess the editing on multiple sites in a sample. You would use multiplex analysis to assess the on- and off-target editing of a guide RNA.